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Addgene

pCAG-CHMP2A-myc, L216D/L219D
(Plasmid #180645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-Myc
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CHMP2A
  • Species
    H. sapiens (human)
  • Mutation
    L216D, L219D
  • Entrez Gene
    CHMP2A (a.k.a. BC-2, BC2, CHMP2, VPS2, VPS2A)
  • Promoter pCAG
  • Tag / Fusion Protein
    • Myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer pCAG for
  • 3′ sequencing primer pCAG rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-CHMP2A-myc, L216D/L219D was a gift from Wesley Sundquist (Addgene plasmid # 180645 ; http://n2t.net/addgene:180645 ; RRID:Addgene_180645)

  • For your References section:

    Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist WI. Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779. 10.7554/eLife.77779 PubMed 36107470
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