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Addgene

Keratin 18 (pcDNA3)
(Plasmid #18064)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Keratin 18
  • Alt name
    KRT18
  • Alt name
    K18
  • Species
    H. sapiens (human)
  • Entrez Gene
    KRT18 (a.k.a. CK-18, CYK18, K18)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Keratin 18 (pcDNA3) was a gift from M Bishr Omary (Addgene plasmid # 18064 ; http://n2t.net/addgene:18064 ; RRID:Addgene_18064)

  • For your References section:

    Identification of the major physiologic phosphorylation site of human keratin 18: potential kinases and a role in filament reorganization. Ku NO, Omary MB. J Cell Biol. 1994 Oct . 127(1):161-71. 10.1083/jcb.127.1.161 PubMed 7523419
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