pCA528 SPASTIN , L177D, residues 108-200
(Plasmid
#180634)
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PurposeBacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCA528
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSPASTIN
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SpeciesH. sapiens (human)
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MutationResidues 108-200, L177D
- Promoter T7
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Tag
/ Fusion Protein
- HIS-SUMO (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 for
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.02.09.477148v1
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCA528 SPASTIN , L177D, residues 108-200 was a gift from Wesley Sundquist (Addgene plasmid # 180634 ; http://n2t.net/addgene:180634 ; RRID:Addgene_180634) -
For your References section:
Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist WI. Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779. 10.7554/eLife.77779 PubMed 36107470
