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pEGFP/golgin-160/3DE
(Plasmid #180631)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180631 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    golgin-160/3DE
  • Alt name
    GOLGA3
  • Alt name
    gcp170
  • Species
    H. sapiens (human)
  • Mutation
    D59E, D139E, D311E
  • Entrez Gene
    GOLGA3 (a.k.a. MEA-2, GCP170)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Caspase-resistant golgin-160

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP/golgin-160/3DE was a gift from Carolyn Machamer (Addgene plasmid # 180631 ; http://n2t.net/addgene:180631 ; RRID:Addgene_180631)

  • For your References section:

    Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis. Mancini M, Machamer CE, Roy S, Nicholson DW, Thornberry NA, Casciola-Rosen LA, Rosen A. J Cell Biol. 2000 May 1;149(3):603-12. doi: 10.1083/jcb.149.3.603. 10.1083/jcb.149.3.603 PubMed 10791974
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