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Addgene

Keratin 8 (pcDNA3)
(Plasmid #18063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Keratin 8
  • Alt name
    KRT8
  • Alt name
    K8
  • Species
    H. sapiens (human)
  • Entrez Gene
    KRT8 (a.k.a. CARD2, CK-8, CK8, CYK8, K2C8, K8, KO)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Keratin 8 (pcDNA3) was a gift from M Bishr Omary (Addgene plasmid # 18063 ; http://n2t.net/addgene:18063 ; RRID:Addgene_18063)

  • For your References section:

    Keratin 8 phosphorylation by p38 kinase regulates cellular keratin filament reorganization: modulation by a keratin 1-like disease causing mutation. Ku NO, Azhar S, Omary MB. J Biol Chem. 2002 Mar 29. 277(13):10775-82. 10.1074/jbc.M107623200 PubMed 11788583
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