pCA528 SPASTIN , residues 108-200
(Plasmid #180628)

• Purpose: Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.
• Depositing Lab: Wesley Sundquist
• Publication: Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779.

Backbone

• Vector backbone: pCA528
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SPASTIN
• Species: H. sapiens (human)
• Mutation: Residues 108-200
• Promoter: T7
• Tag / Fusion Protein:
  • HIS-SUMO (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 for
• 3′ sequencing primer: T7 term

Resource Information

• Supplemental Documents:
  • pCA528-wSPASTIN_108-200.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.02.09.477148v1