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Addgene

pCA528 AMSHLP 16-218
(Plasmid #180609)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180609 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCA528
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AMSHLP residues 16-218
  • Species
    H. sapiens (human)
  • Mutation
    Residues 16-218
  • Promoter T7
  • Tag / Fusion Protein
    • HIS-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BSA1 (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 for
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCA528 AMSHLP 16-218 was a gift from Wesley Sundquist (Addgene plasmid # 180609 ; http://n2t.net/addgene:180609 ; RRID:Addgene_180609)

  • For your References section:

    Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist WI. Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779. 10.7554/eLife.77779 PubMed 36107470
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