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pcDNA3.1-TRBP-L1+D1+L2-myc/His
(Plasmid #180599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRBP
  • Alt name
    TARBP2
  • Alt name
    TAR (HIV-1) RNA binding protein 2
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_134323.2
  • Entrez Gene
    TARBP2 (a.k.a. LOQS, TRBP, TRBP1, TRBP2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer aaagggaagcttatgagtgaagaggagcaaggctccgg
  • 3′ sequencing primer aaaggggcggccgcgcactcagactgctgaggggagacag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-TRBP-L1+D1+L2-myc/His was a gift from Kumiko Ui-Tei (Addgene plasmid # 180599 ; http://n2t.net/addgene:180599 ; RRID:Addgene_180599)

  • For your References section:

    LGP2 virus sensor regulates gene expression network mediated by TRBP-bound microRNAs. Takahashi T, Nakano Y, Onomoto K, Murakami F, Komori C, Suzuki Y, Yoneyama M, Ui-Tei K. Nucleic Acids Res. 2018 Sep 28;46(17):9134-9147. doi: 10.1093/nar/gky575. 10.1093/nar/gky575 PubMed 29939295
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