pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA
(Plasmid
#180590)
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PurposeOpto-cytTrkB
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-hSyn1-DIO-EGFP
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrkB
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SpeciesSynthetic
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MutationPHR(E281A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA was a gift from Won Do Heo (Addgene plasmid # 180590 ; http://n2t.net/addgene:180590 ; RRID:Addgene_180590) -
For your References section:
Optogenetic Modulation of TrkB Signaling in the Mouse Brain. Hong J , Heo WD. J Mol Biol. 2020 Feb 14;432(4):815-827. doi: 10.1016/j.jmb.2020.01.010. Epub 2020 Jan 18. 10.1016/j.jmb.2020.01.010 PubMed 31962123