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Addgene

pAAV-EF1a-pMagHigh-FlpC
(Plasmid #180586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-Ef1a-EGFP
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Split FlpO
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-pMagHigh-FlpC was a gift from Won Do Heo (Addgene plasmid # 180586 ; http://n2t.net/addgene:180586 ; RRID:Addgene_180586)

  • For your References section:

    Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions. Jung H, Kim SW, Kim M, Hong J, Yu D, Kim JH, Lee Y, Kim S, Woo D, Shin HS, Park BO, Do Heo W. Nat Commun. 2019 Jan 18;10(1):314. doi: 10.1038/s41467-018-08282-8. 10.1038/s41467-018-08282-8 PubMed 30659191
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