pCMV-G-Cdc42
(Plasmid
#180577)
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PurposeddGFP sensor of Cdc42
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-EGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameddGFP sensor of Cdc42
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SpeciesH. sapiens (human), Synthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-G-Cdc42 was a gift from Won Do Heo (Addgene plasmid # 180577 ; http://n2t.net/addgene:180577 ; RRID:Addgene_180577) -
For your References section:
Intensiometric biosensors visualize the activity of multiple small GTPases in vivo. Kim J , Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB, Heo WD. Nat Commun. 2019 Jan 14;10(1):211. doi: 10.1038/s41467-018-08217-3. 10.1038/s41467-018-08217-3 PubMed 30643148