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Addgene

pCMV-R-Hras
(Plasmid #180574)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180574 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-EGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ddRFP sensor of Hras
  • Species
    H. sapiens (human), Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-R-Hras was a gift from Won Do Heo (Addgene plasmid # 180574 ; http://n2t.net/addgene:180574 ; RRID:Addgene_180574)

  • For your References section:

    Intensiometric biosensors visualize the activity of multiple small GTPases in vivo. Kim J , Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB, Heo WD. Nat Commun. 2019 Jan 14;10(1):211. doi: 10.1038/s41467-018-08217-3. 10.1038/s41467-018-08217-3 PubMed 30643148
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