pGM-ENO2-mCherry-YFP
(Plasmid
#180570)
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Purpose(Empty Backbone) Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180570 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePTH761
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440232v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGM-ENO2-mCherry-YFP was a gift from Joel McManus (Addgene plasmid # 180570 ; http://n2t.net/addgene:180570 ; RRID:Addgene_180570) -
For your References section:
Unraveling the influences of sequence and position on yeast uORF activity using massively parallel reporter systems and machine learning. May GE, Akirtava C, Agar-Johnson M, Micic J, Woolford J, McManus J. Elife. 2023 May 25;12:e69611. doi: 10.7554/eLife.69611. 10.7554/eLife.69611 PubMed 37227054
Map uploaded by the depositor.
