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Addgene

RGS-SENP5
(Plasmid #18053)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18053 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3/RGS
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SENP5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Entrez Gene
    SENP5
  • Tag / Fusion Protein
    • RGS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RGS-SENP5 was a gift from Edward Yeh (Addgene plasmid # 18053 ; http://n2t.net/addgene:18053 ; RRID:Addgene_18053)

  • For your References section:

    Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3. Gong L, Yeh ET. J Biol Chem. 2006 Jun 9. 281(23):15869-77. 10.1074/jbc.M511658200 PubMed 16608850
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