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Addgene

KTK_136
(Plasmid #180520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA331
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bpi I restriction site removed from replication protein coding gene. MCS replaced with Consitutive GFP drop-out TU with KTK GG overhands for D1.1 cloning. Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KTK_136 was a gift from Tom Ellis (Addgene plasmid # 180520 ; http://n2t.net/addgene:180520 ; RRID:Addgene_180520)

  • For your References section:

    Komagataeibacter Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria. Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T. ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 10.1021/acssynbio.1c00358. Epub 2021 Nov 12. 10.1021/acssynbio.1c00358 PubMed 34767345
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