pcDNA3.1-EGFP(W58stop)-HiBiT
(Plasmid
#180508)
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PurposeMammalian expression of EGFP(W58stop)-HiBiT
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180508 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP(W58stop)
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MutationW58(UGG) to amber stop codon(UAG)
- Promoter CMV
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Tag
/ Fusion Protein
- HiBiT (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477620v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-EGFP(W58stop)-HiBiT was a gift from Masaharu Somiya (Addgene plasmid # 180508 ; http://n2t.net/addgene:180508 ; RRID:Addgene_180508) -
For your References section:
Verification of extracellular vesicle-mediated functional mRNA delivery by RNA editing. Somiya M, Kuroda S. bioRxiv 2022.01.25.477620 10.1101/2022.01.25.477620
