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Addgene

Phleo/Xpress
(Plasmid #1805)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1805 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phleo/Xpress
  • Insert Size (bp)
    922
  • Tag / Fusion Protein
    • Xpress (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Phleo/Xpress was a gift from Christian Tschudi (Addgene plasmid # 1805 ; http://n2t.net/addgene:1805 ; RRID:Addgene_1805)

  • For your References section:

    In vivo epitope tagging of Trypanosoma brucei genes using a one step PCR-based strategy. Shen S, Arhin GK, Ullu E, Tschudi C. Mol Biochem Parasitol 2001 Mar;113(1):171-3. 10.1016/S0166-6851(00)00383-2 PubMed 11254965
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