Skip to main content
Addgene

pCMV-Armc12/FLAG/BioID2/HA
(Plasmid #180498)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180498 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    MCS-BioID2-HA
  • Backbone size w/o insert (bp) 6112
  • Total vector size (bp) 6112
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    armadillo repeat containing 12
  • Alt name
    Armc12
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1020
  • GenBank ID
    NM_026290.3
  • Entrez Gene
    Armc12 (a.k.a. 4930511I11Rik, AV041658)
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • FLAG tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTAATACGACTCACTATAGG
  • 3′ sequencing primer AGCTCACGTTCCACTCCTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Armc12/FLAG/BioID2/HA was a gift from Masahito Ikawa (Addgene plasmid # 180498 ; http://n2t.net/addgene:180498 ; RRID:Addgene_180498)

  • For your References section:

    ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, Matzuk MM, Ikawa M. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6). pii: 2018355118. doi: 10.1073/pnas.2018355118. 10.1073/pnas.2018355118 PubMed 33536340
Related items: