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MSCV-HA tagged-Mouse Mdm2 wt-IRES-GFP
(Plasmid #180491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Mdm2 full length (wt)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • Promoter MSCV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer EcoR!
  • 3′ sequencing primer EcoR1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-HA tagged-Mouse Mdm2 wt-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 180491 ; http://n2t.net/addgene:180491 ; RRID:Addgene_180491)

  • For your References section:

    The RING domain of Mdm2 can inhibit cell proliferation. Dang J, Kuo ML, Eischen CM, Stepanova L, Sherr CJ, Roussel MF. Cancer Res. 2002 Feb 15;62(4):1222-30. PubMed 11861407
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