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pFastBac1 human full-length CENP-E-mNeon-6xHis
(Plasmid #180484)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180484 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastbac 1
  • Backbone manufacturer
    Thermofisher-invitrogen
  • Backbone size w/o insert (bp) 4776
  • Total vector size (bp) 15668
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CENP-E
  • Species
    H. sapiens (human), Synthetic; human
  • Insert Size (bp)
    8103
  • Entrez Gene
    CENPE (a.k.a. CENP-E, KIF10, MCPH13, PPP1R61)
  • Promoter polyhedrin promoter
  • Tag / Fusion Protein
    • mNeon-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pPH
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1 human full-length CENP-E-mNeon-6xHis was a gift from Julie Welburn (Addgene plasmid # 180484 ; http://n2t.net/addgene:180484 ; RRID:Addgene_180484)

  • For your References section:

    Reconstitution of an active human CENP-E motor. Craske B, Legal T, Welburn JPI. Open Biol. 2022 Mar;12(3):210389. doi: 10.1098/rsob.210389. Epub 2022 Mar 9. 10.1098/rsob.210389 PubMed 35259950
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