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p5xQUAS-ZsGreen-P2A, α-cry:mCherry
(Plasmid #180477)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180477 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKY64-miniTol2-opt-CRY-eGFP
  • Backbone manufacturer
    Yun Kee
  • Backbone size w/o insert (bp) 6173
  • Total vector size (bp) 7203
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    5x QUAS-ZsGreen-P2A
  • Species
    Synthetic
  • Insert Size (bp)
    1034

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gtaaaacgacggccagt
  • 3′ sequencing primer atctcaacagtctccaca
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Alt name
    RFP
  • Species
    Synthetic
  • Insert Size (bp)
    711

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer caggtatataaccagggg
  • 3′ sequencing primer GTAACCATTATAAGCTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid construct for binary IQ-Switch transgenesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5xQUAS-ZsGreen-P2A, α-cry:mCherry was a gift from Hyunju Ro (Addgene plasmid # 180477 ; http://n2t.net/addgene:180477 ; RRID:Addgene_180477)

  • For your References section:

    IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish. Hong J, Lee JG, Sohn KC, Lee K, Lee S, Lee J, Hong J, Choi D, Hong Y, Jin HS, Choi DK, Lee SU, Kee Y, Jung J, Bae YK, Hwang RH, Hur GM, Lee JS, Ro H. Commun Biol. 2021 Dec 16;4(1):1405. doi: 10.1038/s42003-021-02923-3. 10.1038/s42003-021-02923-3 PubMed 34916605
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