pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
(Plasmid
#180475)
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PurposeInducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
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Backbone manufacturerHyunju Ro
- Backbone size w/o insert (bp) 7952
- Total vector size (bp) 11440
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZebrafish ubb promoter
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Alt nameUbiquitin B promoter
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3483
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CGAGgtcgacggtatcg
- 3′ sequencing primer GGCGTTGTTGCTGCT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid construct for binary IQ-Switch transgenesis
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP was a gift from Hyunju Ro (Addgene plasmid # 180475 ; http://n2t.net/addgene:180475 ; RRID:Addgene_180475) -
For your References section:
IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish. Hong J, Lee JG, Sohn KC, Lee K, Lee S, Lee J, Hong J, Choi D, Hong Y, Jin HS, Choi DK, Lee SU, Kee Y, Jung J, Bae YK, Hwang RH, Hur GM, Lee JS, Ro H. Commun Biol. 2021 Dec 16;4(1):1405. doi: 10.1038/s42003-021-02923-3. 10.1038/s42003-021-02923-3 PubMed 34916605
