pGL198
(Plasmid
#180472)
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Purpose(Empty Backbone) For expression of Cas9 and gRNAs, multiple gRNAs cloning vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL198
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Vector typePlant Expression, CRISPR
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Tag
/ Fusion Protein
- ZmCas9
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL198 was a gift from Peggy Lemaux (Addgene plasmid # 180472 ; http://n2t.net/addgene:180472 ; RRID:Addgene_180472) -
For your References section:
Morphogene-assisted transformation of Sorghum bicolor allows more efficient genome editing. Aregawi K, Shen J, Pierroz G, Sharma MK, Dahlberg J, Owiti J, Lemaux PG. Plant Biotechnol J. 2021 Nov 26. doi: 10.1111/pbi.13754. 10.1111/pbi.13754 PubMed 34837319