pGL193
(Plasmid
#180471)
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Purpose(Empty Backbone) For expression of Cas9 and gRNAs, multiple gRNAs cloning vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL193
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Vector typePlant Expression, CRISPR
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Tag
/ Fusion Protein
- Cas9
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL193 was a gift from Peggy Lemaux (Addgene plasmid # 180471 ; http://n2t.net/addgene:180471 ; RRID:Addgene_180471) -
For your References section:
Morphogene-assisted transformation of Sorghum bicolor allows more efficient genome editing. Aregawi K, Shen J, Pierroz G, Sharma MK, Dahlberg J, Owiti J, Lemaux PG. Plant Biotechnol J. 2021 Nov 26. doi: 10.1111/pbi.13754. 10.1111/pbi.13754 PubMed 34837319
Map uploaded by the depositor.
