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Addgene

FLAG-SENP2
(Plasmid #18047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFLAG-CMV
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SENP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1770
  • Entrez Gene
    SENP2 (a.k.a. AXAM2, SMT3IP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that compared to GenBank reference sequence NP_067640.2, the SENP2 insert in this plasmid contains a T552A polymorphism.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-SENP2 was a gift from Edward Yeh (Addgene plasmid # 18047 ; http://n2t.net/addgene:18047 ; RRID:Addgene_18047)

  • For your References section:

    SUMO-specific protease 2 is essential for suppression of polycomb group protein-mediated gene silencing during embryonic development. Kang X, Qi Y, Zuo Y, Wang Q, Zou Y, Schwartz RJ, Cheng J, Yeh ET. Mol Cell. 2010 Apr 23. 38(2):191-201. 10.1016/j.molcel.2010.03.005 PubMed 20417598
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