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pAG8H-GB1-EWS_171-264
(Plasmid #180466)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180466 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 5200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EWSR1
  • Alt name
    EWS RNA binding protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    279
  • Mutation
    deleted 1-170, 265-656
  • Entrez Gene
    EWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)
  • Promoter T7
  • Tags / Fusion Proteins
    • His8 (N terminal on backbone)
    • GB1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kasi (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

synthetic, codon optimized for expression in E. coli

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG8H-GB1-EWS_171-264 was a gift from David Libich (Addgene plasmid # 180466 ; http://n2t.net/addgene:180466 ; RRID:Addgene_180466)

  • For your References section:

    The (1)H, (15)N and (13)C resonance assignments of the low-complexity domain from the oncogenic fusion protein EWS-FLI1. Johnson CN, Xu X, Holloway SP, Libich DS. Biomol NMR Assign. 2022 Apr;16(1):67-73. doi: 10.1007/s12104-021-10061-4. Epub 2022 Jan 7. 10.1007/s12104-021-10061-4 PubMed 34994941
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