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Addgene

STR-KDEL_PSAP-SBP-mCherry
(Plasmid #180440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESneo3
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prosaposin
  • Alt name
    PSAP
  • Entrez Gene
    PSAP (a.k.a. GLBA, PARK24, PSAPD, SAP1, SAP2)
  • Tag / Fusion Protein
    • SBP-mCherry (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STR-KDEL_PSAP-SBP-mCherry was a gift from Shawn Ferguson (Addgene plasmid # 180440 ; http://n2t.net/addgene:180440 ; RRID:Addgene_180440)

  • For your References section:

    Efficient progranulin exit from the ER requires its interaction with prosaposin, a Surf4 cargo. Devireddy S, Ferguson SM. J Cell Biol. 2022 Feb 7;221(2):e202104044. doi: 10.1083/jcb.202104044. Epub 2021 Dec 17. 10.1083/jcb.202104044 PubMed 34919127
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