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pU6-pegRNA-LRRK2-G2019S-3a
(Plasmid #180432)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180432 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pU6-pegRNA-GG-acceptor
  • Backbone manufacturer
    Addgene Plasmid #132777
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ncRNA targeting LRRK2-G2019S
  • Alt name
    PARK8; RIPK7; ROCO2; AURA17; DARDARIN
  • gRNA/shRNA sequence
    PBS9-RTT12: GATTGCAAAGATTGCTGACTAgttttAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGgtgcGCAATGCtGTAGTCAGCAATC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    118
  • Mutation
    LRRK2-G2019S
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer ACTATCATATGCTTACCGTAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-pegRNA-LRRK2-G2019S-3a was a gift from Frank Soldner (Addgene plasmid # 180432 ; http://n2t.net/addgene:180432 ; RRID:Addgene_180432)

  • For your References section:

    Highly efficient generation of isogenic pluripotent stem cell models using prime editing. Li H, Busquets O, Verma Y, Syed KM, Kutnowski N, Pangilinan GR, Gilbert LA, Bateup HS, Rio DC, Hockemeyer D, Soldner F. Elife. 2022 Sep 7;11:e79208. doi: 10.7554/eLife.79208. 10.7554/eLife.79208 PubMed 36069759
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