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pαH-S-GSAS-OMICRON
(Plasmid #180423)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180423 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4746
  • Total vector size (bp) 8361
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spike (S-GSAS-OMICRON)
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3615
  • Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; A67V-∆H69/V70-T95I, G142D, V143-, Y144-, Y145-, N211-, L212I, +214EPE-G339D-S371L-S373P-S375F-K417N-N440K-G446S-S477N-T478K-E484A-Q493R-G496S-Q498R-N501Y-Y505H-T547K-D614G-H655Y-N679K-P681H-N764K -D796Y-N856K-Q954H-N969K-L981F
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • HRV3C cleavage site, 8X His tag, 2X Strep-Tag II (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jason McLellan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See this as a reference: https://www.addgene.org/164566/.

Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477784v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-S-GSAS-OMICRON was a gift from Priyamvada Acharya (Addgene plasmid # 180423 ; http://n2t.net/addgene:180423 ; RRID:Addgene_180423)

  • For your References section:

    Structural diversity of the SARS-CoV-2 Omicron spike. Gobeil SM, Henderson R, Stalls V, Janowska K, Huang X, May A, Speakman M, Beaudoin E, Manne K, Li D, Parks R, Barr M, Deyton M, Martin M, Mansouri K, Edwards RJ, Eaton A, Montefiori DC, Sempowski GD, Saunders KO, Wiehe K, Williams W, Korber B, Haynes BF, Acharya P. Mol Cell. 2022 Jun 2;82(11):2050-2068.e6. doi: 10.1016/j.molcel.2022.03.028. Epub 2022 Mar 25. 10.1016/j.molcel.2022.03.028 PubMed 35447081
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