pSL-1
(Plasmid
#180422)
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PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBTK402
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Backbone manufacturerJeffrey Barrick (Addgene plasmid # 110598)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRed Chromprotein gene
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byplasmid pSL1 was built by replacing the RFP dropout in pBTK402 Jeffrey Barrick (Addgene plasmid # 110598) with the E1010 Red Chromprotein gene from part BBa_E1010 from the iGem registry.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSL-1 was a gift from Nancy Moran (Addgene plasmid # 180422 ; http://n2t.net/addgene:180422 ; RRID:Addgene_180422) -
For your References section:
Species divergence in gut-restricted bacteria of social bees. Li Y, Leonard SP, Powell JE, Moran NA. Proc Natl Acad Sci U S A. 2022 May 3;119(18):e2115013119. doi: 10.1073/pnas.2115013119. Epub 2022 Apr 25. 10.1073/pnas.2115013119 PubMed 35467987
