pSL1-E2C
(Plasmid #180421)

• Purpose: Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.
• Depositing Lab: Nancy Moran
• Publication: Li et al Proc Natl Acad Sci U S A. 2022 May 3;119(18):e2115013119. doi: 10.1073/pnas.2115013119. Epub 2022 Apr 25.

Backbone

• Vector backbone: pBTK402
• Backbone manufacturer: Jeffrey Barrick (Addgene plasmid # 110598)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: E2 Crimson

Resource Information

• Supplemental Documents:
  • pSL1-E2C_for_addgene.gb
• A portion of this plasmid was derived from a plasmid made by: plasmid pSL1 was built by replacing the RFP dropout in pBTK402 (Addgene plasmid # 110598) with the E1010 Red Chromprotein gene from part BBa_E1010 from the iGem registry. plasmid pSL1-E2C replaces E1010 with E2-Crimson from plasmid pBTK570 (Addgene plasmid # 110615).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains an 85bp discrepancy downstream of E2 Crimson at the BBa_B0015 Terminator compared to the depositor's provided sequence. This discrepancy is not known to affect plasmid function.