pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10
(Plasmid #180357)

• Purpose: mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11
• Depositing Lab: Manos Mavrakis
• Publication: Martins et al J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 10.1083/jcb.202203016. Epub 2022 Dec 23.

Backbone

• Vector backbone: pTRIP TRE BI
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SEPTIN2
• Alt name: SEPT2
• Species: H. sapiens (human)
• Mutation: SEPT2 F20D, V27D
• Entrez Gene: SEPTIN2 (a.k.a. DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, hNedd5)
• Promoter: bidirectional TRE
• Tag / Fusion Protein:
  • GFP11 (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: SEPTIN 2
• Species: H. sapiens (human)
• Mutation: SEPT2 F20D, V27D
• Entrez Gene: SEPTIN2 (a.k.a. DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, hNedd5)
• Promoter: bidirectional TRE
• Tag / Fusion Protein:
  • GFP10 (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: IRES-TagBFP

Resource Information

• Supplemental Documents:
  • pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.02.23.481653 for bioRxiv preprint.