pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2
(Plasmid
#180353)
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Purposemammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRIP TRE BI
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameSEPTIN7
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Alt nameSEPT7
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SpeciesH. sapiens (human)
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MutationSEPT7 H279D
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Entrez GeneSEPTIN7 (a.k.a. CDC10, CDC3, NBLA02942, SEPT7, SEPT7A)
- Promoter bidirectional TRE
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Tag
/ Fusion Protein
- GFP10 (N terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameSEPTIN7
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Alt nameSEPT7
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SpeciesH. sapiens (human)
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MutationSEPT7 H279D
- Promoter bidirectional TRE
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Tag
/ Fusion Protein
- GFP11 (N terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameIRES-TagBFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.02.23.481653 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2 was a gift from Manos Mavrakis (Addgene plasmid # 180353 ; http://n2t.net/addgene:180353 ; RRID:Addgene_180353) -
For your References section:
Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells. Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S, Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, Koenderink GH, Wenger J, Cabantous S, Mavrakis M. J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 10.1083/jcb.202203016. Epub 2022 Dec 23. 10.1083/jcb.202203016 PubMed 36562751