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pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10
(Plasmid #180349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRIP TRE BI
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SEPTIN7
  • Alt name
    SEPT7
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN7 (a.k.a. CDC10, CDC3, NBLA02942, SEPT7, SEPT7A)
  • Promoter bidirectional TRE
  • Tag / Fusion Protein
    • GFP11 (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI, (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    SEPTIN9
  • Alt name
    SEPT9
  • Alt name
    SEPT9_i3
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN9 (a.k.a. AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1)
  • Promoter bidirectional TRE
  • Tag / Fusion Protein
    • GFP10 (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    IRES-TagBFP

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.02.23.481653 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10 was a gift from Manos Mavrakis (Addgene plasmid # 180349 ; http://n2t.net/addgene:180349 ; RRID:Addgene_180349)

  • For your References section:

    Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells. Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S, Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, Koenderink GH, Wenger J, Cabantous S, Mavrakis M. J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 10.1083/jcb.202203016. Epub 2022 Dec 23. 10.1083/jcb.202203016 PubMed 36562751
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