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Addgene

pCMV_SEPT2 NCmut-mApple
(Plasmid #180321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEPTIN2
  • Alt name
    SEPT2
  • Species
    H. sapiens (human)
  • Mutation
    SEPT2 F20D, V27D
  • Entrez Gene
    SEPTIN2 (a.k.a. DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, hNedd5)
  • Promoter CMV
  • Tag / Fusion Protein
    • mApple (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_SEPT2 NCmut-mApple was a gift from Manos Mavrakis (Addgene plasmid # 180321 ; http://n2t.net/addgene:180321 ; RRID:Addgene_180321)

  • For your References section:

    Septin-microtubule association via a motif unique to the isoform 1 of septin 9 tunes stress fibers. Kuzmic M, Linares GC, Fialova JL, Iv F, Salaun D, Llewellyn A, Gomes M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T, Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P. J Cell Sci. 2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850. 10.1242/jcs.258850 PubMed 34854883
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