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pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep
(Plasmid #180314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pnCS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SEPTIN7
  • Alt name
    SEPT7
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN7 (a.k.a. CDC10, CDC3, NBLA02942, SEPT7, SEPT7A)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    SEPTIN9
  • Alt name
    SEPT9
  • Mutation
    SEPT9_i1-i5
  • Entrez Gene
    SEPTIN9 (a.k.a. AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1)
  • Tag / Fusion Protein
    • TEV-Strep (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mutant is the fusion of the SEPT9_i1-specific N-terminal sequence to SEPT9_i5.

Please note: Plasmid contains a 65bp insertion in the CloDF13 ori. This insertion is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep was a gift from Manos Mavrakis (Addgene plasmid # 180314 ; http://n2t.net/addgene:180314 ; RRID:Addgene_180314)

  • For your References section:

    Septin-microtubule association via a motif unique to the isoform 1 of septin 9 tunes stress fibers. Kuzmic M, Linares GC, Fialova JL, Iv F, Salaun D, Llewellyn A, Gomes M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T, Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P. J Cell Sci. 2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850. 10.1242/jcs.258850 PubMed 34854883
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