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pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6
(Plasmid #180313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pnEA-vH
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, grow at 17degC.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SEPTIN2
  • Alt name
    SEPT2
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN2 (a.k.a. DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, hNedd5)
  • Tags / Fusion Proteins
    • His6-TEV (N terminal on insert)
    • sfCherry2 (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    SEPTIN6
  • Alt name
    SEPT6
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN6 (a.k.a. SEP2, SEPT2, SEPT6)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.02.23.481653 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6 was a gift from Manos Mavrakis (Addgene plasmid # 180313 ; http://n2t.net/addgene:180313 ; RRID:Addgene_180313)

  • For your References section:

    Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells. Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S, Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, Koenderink GH, Wenger J, Cabantous S, Mavrakis M. J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 10.1083/jcb.202203016. Epub 2022 Dec 23. 10.1083/jcb.202203016 PubMed 36562751
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