pLNC Wnt-3aHA
(Plasmid #18030)

• Depositing Lab: Jan Kitajewski
• Publication: Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):1349-58.

Backbone

• Vector backbone: pLNC
• Backbone size w/o insert (bp): 6620
• Vector type: Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a, HB101
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Wnt-3a
• Species: M. musculus (mouse)
• Insert Size (bp): 1300
• Entrez Gene: Wnt3a (a.k.a. Wnt-3a, vt)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: AGCTCGTTTAGTGAACCGTCAGATCG
• 3′ sequencing primer: ACCTACAGGTGGGGTCTTTCATTCCC

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.

Roelink, H., and Nusse, R. Expression of two members of the Wnt family during mouse development -restricted temporal and spatial patterns in the developing neural tube. Genes Dev., 5: 381-388, 1991.