OTUB1/pRetroX-IRES-DsRed#10
(Plasmid
#180279)
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PurposePlasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRetroX-IRES-DsRedExpress Vector
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Backbone manufacturerTAKARA BIO
- Backbone size w/o insert (bp) 5934
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Vector typeMammalian Expression, Retroviral
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Selectable markersDsRed
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman Otubain-1
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Alt nameOTUB1
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SpeciesH. sapiens (human)
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Entrez GeneOTUB1 (a.k.a. HSPC263, OTB1, OTU1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The backbone vector's cat.#632521 from TAKARA BIO
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OTUB1/pRetroX-IRES-DsRed#10 was a gift from Andre Catic (Addgene plasmid # 180279 ; http://n2t.net/addgene:180279 ; RRID:Addgene_180279) -
For your References section:
Imaging-Based Screening of Deubiquitinating Proteases Identifies Otubain-1 as a Stabilizer of c-MYC. Moree SE, Maneix L, Iakova P, Stossi F, Sahin E, Catic A. Cancers (Basel). 2022 Feb 4;14(3). pii: cancers14030806. doi: 10.3390/cancers14030806. 10.3390/cancers14030806 PubMed 35159073