ZsGreen1-cMYC/pLVX-Puromycin
(Plasmid
#180278)
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PurposePlasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180278 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-IRES-Puro
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Backbone manufacturerTAKARA BIO
- Backbone size w/o insert (bp) 8140
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZsGreen1-cMYC fusion protein MYC
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Alt nameGFP-cMYC
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SpeciesH. sapiens (human)
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
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Tag
/ Fusion Protein
- GFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The backbone vector's cat. #632183, from TAKARA BIO
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZsGreen1-cMYC/pLVX-Puromycin was a gift from Andre Catic (Addgene plasmid # 180278 ; http://n2t.net/addgene:180278 ; RRID:Addgene_180278) -
For your References section:
Imaging-Based Screening of Deubiquitinating Proteases Identifies Otubain-1 as a Stabilizer of c-MYC. Moree SE, Maneix L, Iakova P, Stossi F, Sahin E, Catic A. Cancers (Basel). 2022 Feb 4;14(3). pii: cancers14030806. doi: 10.3390/cancers14030806. 10.3390/cancers14030806 PubMed 35159073