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pCasper-pNos-MCP-mCherry
(Plasmid #180275)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180275 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCasper
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 11104
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCP-mCherry
  • Alt name
    MCP-mCherry
  • Insert Size (bp)
    2700
  • Promoter nanos
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAATACTTCAGTTGAATAAACTGTGCTTG
  • 3′ sequencing primer GCCACTTCAACGCTCGATGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCasper-pNos-MCP-mCherry was a gift from Hernan Garcia (Addgene plasmid # 180275 ; http://n2t.net/addgene:180275 ; RRID:Addgene_180275)

  • For your References section:

    Real-time single-cell characterization of the eukaryotic transcription cycle reveals correlations between RNA initiation, elongation, and cleavage. Liu J, Hansen D, Eck E, Kim YJ, Turner M, Alamos S, Garcia HG. PLoS Comput Biol. 2021 May 18;17(5):e1008999. doi: 10.1371/journal.pcbi.1008999. eCollection 2021 May. 10.1371/journal.pcbi.1008999 PubMed 34003867
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