pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64
(Plasmid #180263)

• Purpose: Lentiviral expression vector for dCas9-VP64 in mammalian cells
• Depositing Lab: Alexander Marson
• Publication: Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008. doi: 10.1126/science.abj4008. Epub 2022 Feb 4.

Backbone

• Vector backbone: LentiCRISPRv2
• Total vector size (bp): 13123
• Modifications to backbone: The promoter was switched to SFFV, an mCherry-P2A was introduced upstream of dCas9-VP64, and the sgRNA cassette was removed.
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9
• Species: S. Pyogenes
• Mutation: Nuclease dead Cas9
• Promoter: SFFV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ccaatcagcctgcttctcgc

Resource Information

• Supplemental Documents:
  • pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64.gb
• A portion of this plasmid was derived from a plasmid made by: dCas9-VP64 originated from lentiSAMv2 (Addgene #75112) and cloned into the lentiCRISPRv2-dCas9 backbone (Addgene #112233).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry