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pBa.SBP-NgCAM-GFP
(Plasmid #180247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBa-SBP-eGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NgCAM
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    3750
  • Mutation
    WT
  • GenBank ID
    Z75013
  • Promoter Chicken Beta actin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains A490T and K609E mutations in NgCAM compared to the depositor's provided sequence. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBa.SBP-NgCAM-GFP was a gift from Marvin Bentley (Addgene plasmid # 180247 ; http://n2t.net/addgene:180247 ; RRID:Addgene_180247)

  • For your References section:

    NgCAM and VAMP2 Reveal that Direct Delivery and Dendritic Degradation Maintain Axonal Polarity. Nabb AT, Bentley M. Mol Biol Cell. 2021 Nov 3:mbcE21080425. doi: 10.1091/mbc.E21-08-0425. 10.1091/mbc.E21-08-0425 PubMed 34731031
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