pET21a Lm-iPGM
(Plasmid
#180242)
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PurposeBacterial expression plasmid for production of recombinant Leishmania mexicana iPGM His10
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21a
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 7119
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLeishmania mexicana iPGM-10His
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Alt nameLm iPGM
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Alt nameL.mexicana iPGM-10His
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Alt name2, 3-bisphosphoglycerate-independent phosphoglycerate mutase
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SpeciesLeishmania mexicana
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GenBank IDAJ544274
- Promoter T7
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Hind3 (not destroyed)
- 5′ sequencing primer T7 promotor primer #69348-3
- 3′ sequencing primer T7 terminator primer #69337-3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Lm-iPGM His10 was synthetized by BioBasic and cloned into pET21a using BamH1/Hind3 restriction sites
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21a Lm-iPGM was a gift from James Inglese (Addgene plasmid # 180242 ; http://n2t.net/addgene:180242 ; RRID:Addgene_180242) -
For your References section:
Serum-Stable and Selective Backbone-N-Methylated Cyclic Peptides That Inhibit Prokaryotic Glycolytic Mutases. van Neer RHP, Dranchak PK, Liu L, Aitha M, Queme B, Kimura H, Katoh T, Battaile KP, Lovell S, Inglese J, Suga H. ACS Chem Biol. 2022 Aug 19;17(8):2284-2295. doi: 10.1021/acschembio.2c00403. Epub 2022 Jul 29. 10.1021/acschembio.2c00403 PubMed 35904259