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pET21a Di-iPGM
(Plasmid #180241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 7005
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dirofilaria immitis iPGM-10His
  • Alt name
    Di iPGM
  • Alt name
    D. immitis iPGM-10His
  • Alt name
    2, 3-bisphosphoglycerate-independent phosphoglycerate mutase
  • Species
    Dirofilaria immitis
  • GenBank ID
    HM595362.1
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Hind3 (not destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Di-iPGM His10 was synthetized by BioBasic and cloned into pET21a using BamH1/Hind3 restriction sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a Di-iPGM was a gift from James Inglese (Addgene plasmid # 180241 ; http://n2t.net/addgene:180241 ; RRID:Addgene_180241)

  • For your References section:

    Structure-activity relationship of ipglycermide binding to phosphoglycerate mutases. Wiedmann M, Dranchak PK, Aitha M, Queme B, Collmus CD, Kashipathy MM, Kanter L, Lamy L, Rogers JM, Tao D, Battaile KP, Rai G, Lovell S, Suga H, Inglese J. J Biol Chem. 2021 Apr 1:100628. doi: 10.1016/j.jbc.2021.100628. 10.1016/j.jbc.2021.100628 PubMed 33812994
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