pET303-ColE1-TR-del1-40
(Plasmid #180236)

• Purpose: Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364)
• Depositing Lab: Joanna Slusky
• Publication: Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi: 10.7554/eLife.73297.

Backbone

• Vector backbone: pET303
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5340
• Total vector size (bp): 6342
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Colicin E1
• Alt name: ColE1
• Species: Synthetic; E. coli
• Insert Size (bp): 1002
• Mutation: Truncation of ColE1 containing T and R domains (residues 41-364 ) with deletion of N-terminal 40 residues
• GenBank ID: AAA59418.1
• Promoter: T7
• Tag / Fusion Protein:
  • 6x His-tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/692251 for bioRxiv preprint.