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Addgene

pAC8-TwinStrep-3C-DrHDAC6 (40-831)
(Plasmid #180226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAC8
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone deacetylase 6
  • Alt name
    HDAC6
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2379
  • GenBank ID
    XM_009303751.3
  • Entrez Gene
    hdac6 (a.k.a. wu:fc31d02)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • TwinStrep-3C

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGTTGCTGATATCATGGAGATAATTAAAATG
  • 3′ sequencing primer ACAACGCACAGAATCTAGCGCT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC8-TwinStrep-3C-DrHDAC6 (40-831) was a gift from Patrick Matthias (Addgene plasmid # 180226 ; http://n2t.net/addgene:180226 ; RRID:Addgene_180226)

  • For your References section:

    Expression and Crystallization of HDAC6 Tandem Catalytic Domains. Langousis G, Sanchez J, Kempf G, Matthias P. Methods Mol Biol. 2023;2589:467-480. doi: 10.1007/978-1-0716-2788-4_30. 10.1007/978-1-0716-2788-4_30 PubMed 36255643
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