pEF/myc/ER FLIPglu-170nDelta13V
(Plasmid
#18022)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF/myc/ER
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5603
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLIPglu F16F Delta13
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Alt nameECFP-mglB-Venus
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Alt namePeriplasmic glucose binding
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SpeciesE. coli
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Insert Size (bp)2400
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MutationWT binding protein (no F16A)
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Tags
/ Fusion Proteins
- ECFP (N terminal on insert)
- Venus (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF/myc/ER FLIPglu-170nDelta13V was a gift from Wolf Frommer (Addgene plasmid # 18022 ; http://n2t.net/addgene:18022 ; RRID:Addgene_18022) -
For your References section:
Facilitative plasma membrane transporters function during ER transit. Takanaga H, Frommer WB. FASEB J. 2010 Aug . 24(8):2849-58. 10.1096/fj.09-146472 PubMed 20354141
