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Addgene

pCS2+-dCas9-olEzh2(deltaSET)
(Plasmid #180217)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180217 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 10270
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    enhancer of zeste 2 polycomb repressive complex 2 subunit (deltaSET)
  • Alt name
    Ezh2 (deltaSET)
  • Species
    Oryzias latipes
  • Tags / Fusion Proteins
    • dCas9 (N terminal on insert)
    • FLAG (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+-dCas9-olEzh2(deltaSET) was a gift from Hiroyuki Takeda (Addgene plasmid # 180217 ; http://n2t.net/addgene:180217 ; RRID:Addgene_180217)

  • For your References section:

    Targeted in vivo epigenome editing of H3K27me3. Fukushima HS, Takeda H, Nakamura R. Epigenetics Chromatin. 2019 Mar 13;12(1):17. doi: 10.1186/s13072-019-0263-z. 10.1186/s13072-019-0263-z PubMed 30871638
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