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Addgene

pGB-As-NF-kB2
(Plasmid #180210)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180210 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT9
  • Backbone size w/o insert (bp) 5524
  • Total vector size (bp) 8000
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    As-NF-kB2
  • Alt name
    Acanthoeca spectabilis NF-kB2
  • Species
    Acanthoeca spectabilis
  • Insert Size (bp)
    1224
  • Mutation
    aa 2-502
  • Promoter AdhI
  • Tag / Fusion Protein
    • GAL4 (aa 1-147) fusion protein (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GAL4
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon optimized for human cell expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGB-As-NF-kB2 was a gift from Thomas Gilmore (Addgene plasmid # 180210 ; http://n2t.net/addgene:180210 ; RRID:Addgene_180210)

  • For your References section:

    Comparison of NF-kappaB from the protists Capsaspora owczarzaki and Acanthoeca spectabilis reveals extensive evolutionary diversification of this transcription factor. Williams LM, Sridhar S, Samaroo J, Peart J, Adindu EK, Addanki A, DiRusso CJ, Aguirre Carrion PJ, Rodriguez-Sastre N, Siggers T, Gilmore TD. Commun Biol. 2021 Dec 16;4(1):1404. doi: 10.1038/s42003-021-02924-2. 10.1038/s42003-021-02924-2 PubMed 34916615
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